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Part:BBa_E0035:Design

Designed by: Reshma Shetty   Group: iGEM07_Example   (2007-12-21)


LacZ alpha fragment; complements matching N-terminal deletion mutant (lacZ-omega)


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 93
    Illegal XhoI site found at 144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It has the additional T needed to ensure that the double stop codon is in frame.


Source

This part is derived from BBa_E0033.

References